<table><tr><th>From:</th><th>jeffrey E. &lt;jeevacation@gmail.com&gt;</th></tr><tr><td>Sent:
To:</td><td>Monday, June 23, 2014 5:42 PM
Lesley Groff; Richard Kahn</td></tr><tr><td>Subject:</td><td>Re: Joe Thakuria</td></tr></table>

plese send him my money today.

On Mon, Jun 23, 2014 at=1:33 PM, Lesley Groff rote:

Please see below...do you want to send him the 4 other samples?

Lesley, thanks for the email.

Jeffrey, can add whoever he want into the YPO exome study but I will=need to have those samples mailed to me directly and received by next Mond=y at the latest to be included in this sequencing run.

The exomes (at cost) are $1000 each. I'll add this =o his invoice which I'll send before the end of the week. <=iv>

I don't want this getting lost or held up in HMS or =GH interoffice mail. So, please mail to my home address:

Joe Thakuria

He should have (if I remember =orrectly) 4 extra kits. If he needs more, let me know right away because w='ll be cutting it close in terms of getting into this round (with mail=ng it out to him and then back to me).

The instructions in the pack are straightforward and he=just did it recently but, to summarize:

1. do not=eat/drink/smoke or chew gum for 30minutes prior to collecting (eg, if you =te a chili dog, we could get contamination from pork, beef, chicken, tomat=, kidney bean, and chili pepper genomes; you'd be surprised how much s=milarity there is on the DNA level between very different species)

2. spit into the funnel and fill to the indicated line =not all the way to the top so there's room for #3) and make sure this =sn't filled with bubbles. If there are bubbles it may need to settle a=little so the process typically takes 2 - 5 minutes.

3. There are 2 caps. When done spitting, close the firs= cap (attached to the funnel the person spits into) so that you hear a cli=k and the liquid in that first cap goes into the tube. That liquid needs t= mix with the collected saliva.

4. Then take that first cap off an put the second small=r cap on and close tightly. Make sure the cap is on well and not leaking (=r the sample can't be used).

The following ar= VERY IMPORTANT:

5. You or someone else will need to send me the names and contact info=(especially email and phone) for people who are submitting samples. <=div>

	6. But do not put those names on the tubes or fill=out paperwork that came with the tubes. I'll take care of that.

	7. You do need to label the tubes though. For the addit=onal 4 samples, you can use the following designations: YPO 76, =PO 77, YPO 78, YPO 79

8. Obviously I will need to=know who is who. (So you'll need to email me: "YPO 76 is jane doe= YPO 77 is john doe, etc.)

I can't overemphasize the importance of the labelin=. If I'm given the YPO number, names, and/or samples are incorrectly m=tched, the people will get results that are not theirs.

It's actually very straightforward but I want to avoid any m=stakes by spelling everything out.

You can mail t=e tubes back in the boxes (and unfilled paperwork) they came in. But, of c=urse, make sure each tube is labeled with the appropriate YPO St so we know=who is who.

Email me with any questions.

T=anks!
Joe

On Mon, Jun 23, 2014 at 11:29 AM, Lesley Groff < > wrote:

HI Joe...I see you wan=ed to provide Jeffrey with some instruction on the kits today...Let me kno=! thanks, Lesley



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